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The amplifications were performed in a Elucidation of such a genetic variation at polymerase Genei, Bangalore. Amplifications were phenotypic level could be better characterized by performed in a Gene Pro Thermocycler Bioer Tech.
Therefore, the present investigation was Co. The amplified products were loaded in agarose gel 1. The amplifications were checked for their and Branchard, and Ye chunjiang et al. The success in generating wide range of polymorphic loci depends The gels were documented by gel doc system Fire on proper choice of primers for DNA amplification.
The size of amplicons were determined by differentiate two or more cultivars may vary with the comparing with the lambda DNA ladder bp test materials used. When the variation in the with known fragment sizes. Gels were scored for the cultivars is high the use of few primers can serve the presence 1 or absence 0 of bands and the binary purpose of generating useful information.
The mutant produce any amplified products. As a result, thirteen cultures e. High wide array of PCR products in terms of amplicon size yield performance of these mutants could be bp was revealed.
Comparatively, RAPD produced attributed to high tiller number, more no. Whereas, ISSR only. The total number of bands for all primers was potent enough to reveal 60 polymorphic bands ranged from with an average of 5.
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